Journal of Lipid Research, Vol. 15, 273-280, May 1974
Copyright © 1974 by Lipid Research, Inc.
Permeability changes induced by peroxidation in liposomes prepared from human erythrocyte lipids
James E. Smolen and Stephen B. Shohet
Divisions of Hematology, Departments of Medicine and Laboratory Medicine, Moffitt Hospital, University of California, San Francisco Medical Center, San Francisco, California 94143, and Department of Medicine, Children's Hospital Medical Center, Harvard Medical School, Boston, Massachusetts 02115
The diffusion of [2-14C]glucose out of liposomes prepared from extracted human erythrocyte lipids was examined. Increased glucose efflux was observed when the lipids were treated with hydrogen peroxide and CuCl2 before liposome formation, and this phenomenon required both peroxide and metal. Peroxidation of these lipids also resulted in the destruction of polyunsaturated fatty acids and the generation of conjugated dienes, but neither of these processes appeared to be the sole cause of increased glucose efflux. Thin-layer chromatography and the effects of aqueous washes suggested that surface-active lysophosphatides or other lipid degradation products were responsible for the increased permeability of the treated liposomes. It is suggested that the behavior of this liposome model system may be relevant to the high permeability and fragility of vitamin E-deficient erythrocytes.
Supplementary key words glucose efflux
Submitted on October 22, 1972
Revised on September 10, 1973
Accepted on February 11, 1974
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