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Journal of Lipid Research, Vol. 15, 283-285, May 1974
Copyright © 1974 by Lipid Research, Inc.

Quantitative determination of lysolecithin and sphingomyelin in phospholipid mixtures by thin-layer chromatography as applied to the thymus of the fmfm mouse

Linda M. Hoffman , Wai Fok , and Larry Schneck

Department of Neurology of the Isaac Albert Research Institute, Kingsbrook Jewish Medical Center, Brooklyn, New York 11203

Lysolecithin and sphingomyelin may be readily separated from other phospholipids on thin-layer plates impregnated with silver nitrate using the solvent system chloroform-methanol-concentrated ammonium hydroxide 65:35:8. Quantitation is carried out by determining the phosphorus content of the developed bands. The minimum amount of phospholipid that can be quantitatively determined is that which contains 0.7 µg of phosphorus. This method has been used for the determination of lysolecithin and sphingomyelin in the tissues of mice having foam-cell reticulosis.

Supplementary key words phosphorus • argentation chromatography • foam-cell reticulosis • Niemann-Pick disease

Submitted on August 22, 1973
Accepted on January 14, 1974

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