| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Lipid Research, Vol. 15, 286-291, May 1974
The Lipoprotein Research Laboratory and the General Clinical Research Center, the Basic Science Laboratory of the Veterans Administration Hospital, and the Departments of Medicine and Biological Chemistry, University of Cincinnati College of Medicine, Cincinnati, Ohio
A gas-liquid chromatographic micromethod for quantitation of cholesterol in 20 µl of plasma was developed using 5 Supplementary key words gas-liquid chromatography • cholesterol-cholestane peak height ratios
Submitted on October 16, 1973
Copyright © 1974 by Lipid Research, Inc.
Quantitative analysis of cholesterol in 5 to 20 µl of plasma
-cholestane as an internal standard, saponification with tetramethylammonium hydroxide-isopropanol, and extraction with tetrachloroethylene-methyl butyrate. Cholesterol levels in plasma samples were calculated by comparing cholesterol-cholestane peak height ratios with those of preassayed reference plasma. Over a plasma cholesterol range of 44 to 468 mg/100 ml, the gas-liquid chromatographic micromethod and the automated ferric chloride colorimetric method gave nearly identical results (r = 0.99) in duplicate aliquots of 131 plasma samples.
Accepted on February 4, 1974
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  P. G. Yancey, W. G. Jerome, H. Yu, E. E. Griffin, B. E. Cox, V. R. Babaev, S. Fazio, and M. F. Linton Severely altered cholesterol homeostasis in macrophages lacking apoE and SR-BI J. Lipid Res., May 1, 2007; 48(5): 1140 - 1149. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Zhao, J. Song, R. W. St. Clair, and S. Ghosh Stable overexpression of human macrophage cholesteryl ester hydrolase results in enhanced free cholesterol efflux from human THP1 macrophages Am J Physiol Cell Physiol, January 1, 2007; 292(1): C405 - C412. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Qin, T. Nagao, I. Grosheva, F. R. Maxfield, and L. M. Pierini Elevated Plasma Membrane Cholesterol Content Alters Macrophage Signaling and Function Arterioscler. Thromb. Vasc. Biol., February 1, 2006; 26(2): 372 - 378. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. R. Bates, J.-Q. Tao, H. L. Collins, O. L. Francone, and G. H. Rothblat Pulmonary abnormalities due to ABCA1 deficiency in mice Am J Physiol Lung Cell Mol Physiol, December 1, 2005; 289(6): L980 - L989. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. E. Griffin, J. C. Ullery, B. E. Cox, and W. G. Jerome Aggregated LDL and lipid dispersions induce lysosomal cholesteryl ester accumulation in macrophage foam cells J. Lipid Res., October 1, 2005; 46(10): 2052 - 2060. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. J. Edwards, I. M. Berquin, H. Sun, J. T. O'Flaherty, L. W. Daniel, M. J. Thomas, L. L. Rudel, R. L. Wykle, and Y. Q. Chen Differential Effects of Delivery of Omega-3 Fatty Acids to Human Cancer Cells by Low-Density Lipoproteins versus Albumin Clin. Cancer Res., December 15, 2004; 10(24): 8275 - 8283. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. G. Romanenko, Y. Fang, F. Byfield, A. J. Travis, C. A. Vandenberg, G. H. Rothblat, and I. Levitan Cholesterol Sensitivity and Lipid Raft Targeting of Kir2.1 Channels Biophys. J., December 1, 2004; 87(6): 3850 - 3861. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Li, M. Ge, L. Ciani, G. Kuriakose, E. J. Westover, M. Dura, D. F. Covey, J. H. Freed, F. R. Maxfield, J. Lytton, et al. Enrichment of Endoplasmic Reticulum with Cholesterol Inhibits Sarcoplasmic-Endoplasmic Reticulum Calcium ATPase-2b Activity in Parallel with Increased Order of Membrane Lipids: IMPLICATIONS FOR DEPLETION OF ENDOPLASMIC RETICULUM CALCIUM STORES AND APOPTOSIS IN CHOLESTEROL-LOADED MACROPHAGES J. Biol. Chem., August 27, 2004; 279(35): 37030 - 37039. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. G. Romanenko, G. H. Rothblat, and I. Levitan Sensitivity of Volume-regulated Anion Current to Cholesterol Structural Analogues J. Gen. Physiol., December 29, 2003; 123(1): 77 - 88. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ghosh, R. W. St. Clair, and L. L. Rudel Mobilization of cytoplasmic CE droplets by overexpression of human macrophage cholesteryl ester hydrolase J. Lipid Res., October 1, 2003; 44(10): 1833 - 1840. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T. Lada, L. L. Rudel, and R. W. St. Clair Effects of LDL enriched with different dietary fatty acids on cholesteryl ester accumulation and turnover in THP-1 macrophages J. Lipid Res., April 1, 2003; 44(4): 770 - 779. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. G. Romanenko, G. H. Rothblat, and I. Levitan Modulation of Endothelial Inward-Rectifier K+ Current by Optical Isomers of Cholesterol Biophys. J., December 1, 2002; 83(6): 3211 - 3222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T. Lada, M. C. Willingham, and R. W. St. Clair Triglyceride depletion in THP-1 cells alters cholesteryl ester physical state and cholesterol efflux J. Lipid Res., April 1, 2002; 43(4): 618 - 628. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. G. Yancey and W. G. Jerome Lysosomal cholesterol derived from mildly oxidized low density lipoprotein is resistant to efflux J. Lipid Res., March 1, 2001; 42(3): 317 - 327. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. Georgopoulos, J. P. Bantle, M. Noutsou, and H. A. Hoover A High Carbohydrate versus a High Monounsaturated Fatty Acid Diet Lowers the Atherogenic Potential of Big VLDL Particles in Patients with Type 1 Diabetes J. Nutr., October 1, 2000; 130(10): 2503 - 2507. [Abstract] [Full Text]
|
|
|
|
|
|
B. K. Nordskog, J. W. Reagan , Jr., and R. W. St. Clair Sterol synthesis is up-regulated in cholesterol-loaded pigeon macrophages during induction of cholesterol efflux J. Lipid Res., October 1, 1999; 40(10): 1806 - 1817. [Abstract] [Full Text]
|
|
|
|
|
|
A. Rodriguez, P. S. Bachorik, and S.-B. Wee Novel Effects of the Acyl-Coenzyme A:Cholesterol Acyltransferase Inhibitor 58-035 on Foam Cell Development in Primary Human Monocyte–Derived Macrophages Arterioscler. Thromb. Vasc. Biol., September 1, 1999; 19(9): 2199 - 2206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Avart, D. W. Bernard, W. G. Jerome, and J. M. Glick Cholesteryl ester hydrolysis in J774 macrophages occurs in the cytoplasm and lysosomes J. Lipid Res., March 1, 1999; 40(3): 405 - 414. [Abstract] [Full Text]
|
|
|
|
|
|
J. E. Phillips, W. V. Rodrigueza, and W. J. Johnson Basis for rapid efflux of biosynthetic desmosterol from cells J. Lipid Res., December 1, 1998; 39(12): 2459 - 2470. [Abstract] [Full Text]
|
|
|
|
|
|
L. Kritharides, A. Christian, G. Stoudt, D. Morel, and G. H. Rothblat Cholesterol Metabolism and Efflux in Human THP-1 Macrophages Arterioscler. Thromb. Vasc. Biol., October 1, 1998; 18(10): 1589 - 1599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. G. Yancey and W. G. Jerome Lysosomal sequestration of free and esterified cholesterol from oxidized low density lipoprotein in macrophages of different species J. Lipid Res., July 1, 1998; 39(7): 1349 - 1361. [Abstract] [Full Text]
|
|
|
|
|
|
W. G. Jerome, C. Cash, R. Webber, R. Horton, and P. G. Yancey Lysosomal lipid accumulation from oxidized low density lipoprotein is correlated with hypertrophy of the Golgi apparatus and trans-Golgi network J. Lipid Res., July 1, 1998; 39(7): 1362 - 1371. [Abstract] [Full Text]
|
|
|
|
|
|
W. V. Rodrigueza, K. D. Mazany, A. D. Essenburg, M. E. Pape, T. J. Rea, C. L. Bisgaier, and K. J. Williams Large Versus Small Unilamellar Vesicles Mediate Reverse Cholesterol Transport In Vivo Into Two Distinct Hepatic Metabolic Pools : Implications for the Treatment of Atherosclerosis Arterioscler. Thromb. Vasc. Biol., October 1, 1997; 17(10): 2132 - 2139. [Abstract] [Full Text]
|
|
|
|
|
|
S. R.W. St Clair Effect of 17ß-Estradiol on Metabolism of Acetylated Low-Density Lipoprotein by THP-1 Macrophages in Culture Arterioscler. Thromb. Vasc. Biol., September 1, 1997; 17(9): 1691 - 1700. [Abstract] [Full Text]
|
|
|
|
|
|
W. V. Rodrigueza, K. J. Williams, G. H. Rothblat, and M. C. Phillips Remodeling and Shuttling: Mechanisms for the Synergistic Effects Between Different Acceptor Particles in the Mobilization of Cellular Cholesterol Arterioscler. Thromb. Vasc. Biol., February 1, 1997; 17(2): 383 - 393. [Abstract] [Full Text]
|
|
|
|
|
|
P. G. Yancey, W. V. Rodrigueza, E. P.C. Kilsdonk, G. W. Stoudt, W. J. Johnson, M. C. Phillips, and G. H. Rothblat Cellular Cholesterol Efflux Mediated by Cyclodextrins. DEMONSTRATION OF KINETIC POOLS AND MECHANISM OF EFFLUX J. Biol. Chem., July 5, 1996; 271(27): 16026 - 16034. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Sulistiyani, S. J. Adelman, A. Chandrasekaran, J. Jayo, and R. W. St. Clair Effect of 17{alpha}-Dihydroequilin Sulfate, a Conjugated Equine Estrogen, and Ethynylestradiol on Atherosclerosis in Cholesterol-Fed Rabbits Arterioscler. Thromb. Vasc. Biol., July 1, 1995; 15(7): 837 - 846. [Abstract] [Full Text]
|
|
|
|
|
|
G. J. Warner, G. Stoudt, M. Bamberger, W. J. Johnson, and G. H. Rothblat Cell Toxicity Induced by Inhibition of Acyl Coenzyme A:Cholesterol Acyltransferase and Accumulation of Unesterified Cholesterol J. Biol. Chem., March 17, 1995; 270(11): 5772 - 5778. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Seo, M. Al-Haideri, E. Treskova, T. S. Worgall, Y. Kako, I. J. Goldberg, and R. J. Deckelbaum Lipoprotein Lipase-mediated Selective Uptake from Low Density Lipoprotein Requires Cell Surface Proteoglycans and Is Independent of Scavenger Receptor Class B Type 1 J. Biol. Chem., September 22, 2000; 275(39): 30355 - 30362. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
