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Journal of Lipid Research, Vol. 15, 291-294, May 1974
Copyright © 1974 by Lipid Research, Inc.

Simple assay for monoacylglycerol hydrolase activity of rat adipose tissue

Hans Tornqvist , Lennart Krabisch , and Per Belfrage

Department of Physiological Chemistry, Medical Faculty, University of Lund, Lund, Sweden

A simple, sensitive, and specific assay for monoacylglycerol hydrolase activity of rat adipose tissue is described. Monoacyl[³H]glycerols with different chain lengths (8-18 carbon atoms) and different degrees of unsaturation in mixed micellar solution with different detergents can be used as substrates. The [³H]glycerol that is produced is isolated in a one-step liquid-liquid partition procedure. For routine purposes monooleoyl[³H]glycerol was found to be the most suitable substrate. A simple method for the chemical synthesis and purification of this substrate in high yield is given. The assay allows rapid serial sampling of enzymatic activity with a high reproducibility.

Supplementary key words monooleoyl[³H]glycerol synthesis • sodium mono[³H]glycerate • oleoyl chloride

Submitted on June 28, 1973
Accepted on February 6, 1974

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