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Journal of Lipid Research, Vol. 15, 375-379, July 1974
Copyright © 1974 by Lipid Research, Inc.

Biosynthesis of retinal in bovine corpus luteum

Anthony M. Gawienowski , Maria Stacewicz-Sapuncakis , and Raymond Longley

Department of Biochemistry, University of Massachusetts, Amherst, Massachusetts 01002

Bovine corpus luteum tissue was sliced and incubated with ßbeta;-[15,15'-³H]carotene. The conversion of radioactive ßbeta;-carotene into radioactive retinal was substantiated utilizing column chromatography, thin-layer chromatography, high-speed liquid chromatography, and a derivative formation. Lowering of the incubation temperature to 20°C or boiling the tissue eliminated the conversion of ßbeta;-carotene to retinal. In addition, other carotenoids and possible oxidation products of ßbeta;-carotene in the corpus luteum were investigated. Our results indicate that the bovine corpus luteum possesses the ability to synthesize retinal in situ, which may play a role in reproductive functions.

Supplementary key words vitamin A • ßbeta;-carotene • oxidation of carotenoids • dioxygenase • high-speed liquid chromatography

Submitted on December 6, 1973
Accepted on April 4, 1974

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