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Journal of Lipid Research, Vol. 15, 465-473, September 1974
Graduate School of Nutrition and Section of Biochemistry, Molecular and Cell Biology, Division of Biological Sciences, Cornell University, Ithaca, New York 14850
Several methods for measuring cholesterol absorption in the rat have been compared. After administration of an oral dose of labeled cholesterol (14C or 3H) and an intravenous dose of colloidal labeled cholesterol (3H or 14C) the ratio of the two labels in plasma or whole blood 48 hr or more after dosing compared closely to the ratio of areas under the respective specific activity-time curves. The area ratio method is independent of a time lag between the appearance of oral and intravenous label in the bloodstream. Both measures of cholesterol absorption agree fairly well with a method based on measuring the unabsorbed dietary cholesterol in a pooled fecal sample. The plasma isotope ratio method gave more reproducible results than the fecal collection method when the measurement was repeated in the same animals 5 days after the first measurement. Cholesterol absorption was overestimated by the use of Tween 20-solubilized labeled cholesterol for the intravenous dose. The plasma disappearance curves of injected labeled colloidal cholesterol and cholesterol-labeled chylomicrons infused intravenously over a 3.5-hr period in the same animal coincided within experimental error from the first day until 75 days after injection. The plasma isotope ratio method for cholesterol absorption gave the same results in rats practicing coprophagy as in those in which this practice was prevented. The addition of sulfaguanidine to the diet lowered cholesterol absorption as measured by the plasma isotope ratio to the same degree as that measured by the fecal collection method. Supplementary key words fecal labeled sterols • colloidal cholesterol • Tween 20-solubilized cholesterol • production rates • sulfaguanidine • chylomicrons
Submitted on January 16, 1974
Copyright © 1974 by Lipid Research, Inc.
Validation of a dual-isotope plasma ratio method for measurement of cholesterol absorption in rats
Accepted on April 19, 1974
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