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Journal of Lipid Research, Vol. 15, 525-527, September 1974
Copyright © 1974 by Lipid Research, Inc.

A method for determination of saturated phosphatidylcholine

Tadashi Shimojo , Masao Abe , and Masaru Ohta

Department of Biochemistry, Sapporo Medical College, Sapporo, Japan

Phosphatidylcholine preparations containing saturated and unsaturated molecular species were subjected to KMnO₄-NaIO₄ oxidation in aqueous acetic acid, which left only disaturated species intact. After the oxidation, the remaining intact phosphatidylcholine was separated by thin-layer chromatography. The procedure could be used as a simple and rapid method for microdetermination of the saturated species in phosphatidylcholine preparations containing more than 0.1 µmole of the saturated species. The contents of the saturated species in native phosphatidylcholines obtained from rat lung tissue and washings by this procedure were 35.7% and 58.3%, respectively.

Supplementary key words dipalmitoyl lecithin • KMnO₄-NaIO₄ oxidation • thin-layer chromatography

Submitted on December 31, 1973
Accepted on April 22, 1974

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