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Journal of Lipid Research, Vol. 15, 530-532, September 1974
Copyright © 1974 by Lipid Research, Inc.

Tritiated bile acids: problems and recommendations

D. K. Panveliwalla , D. Pertsemlidis , and E. H. Ahrens Jr.

The Rockefeller University, New York 10021, and Mount Sinai School of Medicine, City University of New York, New York 10029

Kinetic studies of cholic and chenodeoxycholic acids were carried out in three patients by simultaneous intravenous administration of appropriate pairs of ³H- and ¹⁴C-labeled compounds. The results obtained indicated two sources of error: chemical impurity and loss of tritium by biological exchange. Precautions are listed for use of tritiated bile acids in studies of pool sizes and turnover rates.

Supplementary key words [2,4-³H]cholic acid • [random-³H]chenodeoxycholic acid • purity • tritium exchange • kinetics

Submitted on September 19, 1973
Revised on January 3, 1974
Accepted on May 6, 1974

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