HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Steinberg, J. Articles by Pal Yu, B. Search for Related Content PubMed PubMed Citation Articles by Steinberg, J. Articles by Pal Yu, B. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 15, 537-543, November 1974
Copyright © 1974 by Lipid Research, Inc.

Role of sarcoplasmic reticulum phospholipids in calcium ion binding activity

Joel Steinberg , E. J. Masoro , and Byung Pal Yu

Department of Physiology and Biophysics, The Medical College of Pennsylvania, Philadelphia, Pennsylvania 19129

The relationship between sarcoplasmic reticulum phospholipid and Ca²⁺ binding by sarcoplasmic reticulum membranes was explored. Ca²⁺ bound in the absence of ATP was defined as "ATP-independent Ca²⁺ binding," and the additional amount of Ca²⁺ bound in the presence of ATP was defined as "ATP-dependent Ca²⁺ binding." The latter was found to be very sensitive to the loss of sarcoplasmic reticulum phospholipid; the amount of Ca²⁺ bound was reduced when as little as 3% of the phospholipid was destroyed by phospholipase C. Further destruction of membrane phospholipid up to a 40% loss caused little or no further reduction of this Ca²⁺ binding. However, when the destruction of phospholipid exceeded 40%, further loss of this Ca²⁺ binding occurred, and there was an almost complete loss of this function when more than 60% of the sarcoplasmic reticulum phospholipid was destroyed.

Supplementary key words phospholipase C • "ATP-dependent Ca²⁺ binding" • "ATP-independent Ca²⁺ binding" • "ATP-dependent, oxalate-promoted Ca²⁺ transport"

Submitted on January 11, 1974
Accepted on June 17, 1974

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?