Journal of Lipid Research, Vol. 15, 557-562, November 1974
Copyright © 1974 by Lipid Research, Inc.
Stimulation of erythroblast maturation in vitro by sphingolipids
R. B. Clayton , J. M. Cooper , Tore Curstedt , Jan Sjövall , Henry Borsook , J. Chin , and A. Schwarz
Department of Psychiatry, Stanford Medical Center, Stanford California 94305; Kemiska Institutionen, Karolinska Institutet, S-10401 Stockholm, Sweden; and University of California, Space Sciences Laboratory, Berkeley, California 94720
A lipid factor previously isolated from leukocytes and found to stimulate basophilic erythroblast formation in an in vitro system of incubated rabbit bone marrow cells has been analyzed by thin-layer chromatography, gas-liquid chromatography, and gas-liquid chromatography-mass spectrometry. The biologically active components are sphingosine ceramides of tetracosanoic and dehydrotetracosanoic acids. Tests of a series of related ceramides show a high degree of structural specificity for the C24-V-acyl compounds with significant but markedly lower activity of the C22 analog. Commercially available sphingomyelin shows activity comparable to that of the tetracosanoic acid ceramide. Sphingosine and tetracosanic acid supplied in equimolar amounts have negligible activity. The results, in the context of other findings, suggest a possible supportive role of plasma ceramides and sphingomyelins in red cell maturation.
Supplementary key words erythrocyte maturation in vitro • basophilic erythroblasts • tetracosanoic (lignoceric) acid ceramides • sphingomyelin • erythropoiesis
Submitted on May 3, 1974
Accepted on July 2, 1974
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