Journal of Lipid Research, Vol. 15, 611-614, November 1974
Copyright © 1974 by Lipid Research, Inc.
Analysis of 
-lipoprotein cholesterol in 50 µl of plasma
R. F. Lutmer , D. Parsons , C. J. Glueck , J. A. Morrison , L. Stewart , J. B. Brazier , C. R. Buncher , and T. T. Ishikawa
Lipoprotein Research Laboratory and the General Clinical Research Center, University of Cincinnati College of Medicine, Cincinnati, Ohio 45219
A micromethod for quantitation of 
-lipoprotein cholesterol was devised for gas-liquid chromatography to minimize plasma sample size and facilitate lipid studies using capillary blood from children or small animals. -Lipoprotein cholesterol was measured by gas-liquid chromatography in 20 µl of the centrifuged supernate obtained after addition of 5 µl of mixed heparin-manganese chloride solution 1:1 (v/v) to 50 µl of plasma. Comparison of venous -lipoprotein cholesterol measured by gas-liquid chromatography with venous -lipoprotein cholesterol measured by conventional heparin-manganese precipitation and ferric chloride (colorimetric) cholesterol determination gave a correlation coefficient of 0.98 for 80 plasma samples. Capillary -lipoprotein cholesterol and venous -lipoprotein cholesterol were closely correlated in 31 patients (r = 0.97).
Supplementary key words gas-liquid chromatography
Submitted on April 1, 1974
Accepted on July 25, 1974
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