Journal of Lipid Research, Vol 16, 116-122, Copyright © 1975 by Lipid Research, Inc.
Demonstration of two pools of albumin-bound fatty acids
C Soler-Argilaga, R Infante and J Polonovski
The uptakes of albumin-bound nonesterified fatty acids and of [1-
14C]palmitic acid complexed to albumin by the isolated perfused rat liver
were compared. During perfusion, the rate of uptake of nonesterified fatty
acids decreased and became zero when the fatty acid:albumin molar ratio
reached 0.3, but the rate of uptake of radioactive palmitic acid remained
constant. This finding suggests the existence of two pools of fatty acids
bound to albumin with different fractional turnover rates. This view was
supported by the fact that when delipidated albumin complexed in vitro to
radioactive and nonradioactive fatty acids was used no difference was
observed between the uptakes of nonesterified fatty acids and radioactive
fatty acids by perfused liver. Similar results were found with
albumin-bound radioactive fatty acid in vivo (obtained from rats fed
radioactive palmitic acid), showing a homogeneous distribution of the label
in both pools. The existence of two nonesterified fatty acid pools in
plasma would arise from the differences in the nature of bonds between
fatty acid and albumin molecules, which could determine the rate of
exchange of fatty acids between the albumin-bound and soluble forms
preceding their uptake by the cells.
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