Journal of Lipid Research, Vol 16, 155-158, Copyright © 1975 by Lipid Research, Inc.
Determination of bile acid pool size in man: a simplified method with advantages of increases precision, shortened analysis time, and decreased isotope exposure
WC Duane, RD Adler, LJ Bennion and RL Ginsberg
A simplified isotope dilution method for measurement of the bile acid pool
size in normal subjects is described and compared with the traditional
method of Lindstedt (Acta Physiol. Scand. 40: 1-9, 1957). Advantages of
this simplified method include a four- to eightfold reduction of isotope
dose, facilitation of analytical procedures, and a reduction in the
required number of duodenal intubations. In 15 human subjects who had two
separate estimates of pool size by this method, precision averaged 2.6
percent. In 16 comparisons, pool size measured by this method averaged 13.7
percent higher than simultaneous estimates by the Lindstedt method. Factors
affecting accuracy (as opposed to precision) in both methods are discussed.
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