Journal of Lipid Research, Vol 16, 392-394, Copyright © 1975 by Lipid Research, Inc.
A simplified procedure for synthesis of di-[14C]acyl-labeled lecithins
EL Pugh and M Kates
A simplified procedure for synthesis of 1,2-di-[1'-14C]oleoyl-, 1,2-di-
[1'-14C]linoleoyl-, and 1,2-di-[1'-14C]eicosatrienoyl-sn-glycero-3-
phosphorylcholine is described. The method involves acylation of the CdCl2
complex of glycerophosphorylcholine with a 14C-labeled fatty acid in the
presence of trifluoracetic anhydride and pyridine. The 14C- labeled
lecithin is isolated in pure form by preparative thin-layer chromatography
and alumina column chromatography in an overall yield of 12-24%. No
isomerization or peroxidation of the unsaturated acids was detected.
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