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Journal of Lipid Research, Vol 16, 441-447, Copyright © 1975 by Lipid Research, Inc.
Different populations of pig epidermal cells: isolation and lipid composition
GM Gray and HJ Yardley
Preparations representing populations of (a) basal and spinous cells, (b)
granular cells, and (c) stratum corneum cells were obtained by successive
treatments of epidermal slices from pig skin with dilute buffered trypsin
solutions. Total lipids accounted for about 8% of the cell dry weight in
each of the three populations. Phospholipids, which predominated in the
basal and spinous cells, accounted for only 21% of the total lipids in the
granular cells and less than 0.1% in the stratum corneum. The latter cells
contained more cholesterol (23% of total lipid) than either the granular
cells (18%) or the basal and spinous cells (8%). The proportion of ceramide
was also much higher in the stratum corneum (17%) and granular cells (9%)
than in the basal and spinous cells (1%). The relative amounts of
glycosphingolipid (glucosylceramide) and cholesteryl sulfate in the total
lipids of stratum corneum cells were less than half those in the granular
cells and basal and spinous cells. A novel phospholipid was a major
component (26% of total) of the phospholipids from granular cells. The
compound, which was partially characterized, contained phosphorus, fatty
acids, and glycerol (molar ratio 1:3:2) and appeared to be a neutral
derivative of phosphatidic acid.

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Copyright © 1975 by the American Society for Biochemistry and Molecular Biology.
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