J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
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Journal of Lipid Research, Vol. 16, 448-453, November 1975
Copyright © 1975 by Lipid Research, Inc.

High-pressure liquid chromatography: separation of the metabolites of vitamins D2 and D3 on small-particle silica columns

Glenville Jones and Hector F. DeLuca

Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706

The high-pressure liquid chromatographic separation of all of the known metabolities of vitamin D2 and vitamin D3 found in biological fluids has been achieved. This technique has been successfully applied to the analysis of vitamin D mixtures, purification of vitamin D metabolities, and identification of radioactive peaks. Some theoretical bases for the observed resolutions are suggested.

Supplementary key words 1,25-dihydroxyvitamin D3 • 25-hydroxyvitamin D3

Submitted on March 24, 1975
Accepted on July 14, 1975


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