HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Schonfeld, G. Articles by Bailey, A. P. Search for Related Content PubMed PubMed Citation Articles by Schonfeld, G. Articles by Bailey, A. P. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 17, 25-29, Copyright © 1976 by Lipid Research, Inc.

ARTICLES

Measurement of apolipoprotein A-I in rat high density lipoprotein and in rat plasma by radioimmunoassay

G Schonfeld, MS Frick and AP Bailey

A double antibody radioimmunoassay (RIA) for rat apolipoprotein A-I is reported. The ApoA-I isolated from delipidated HDL by gel filtration yielded a single band on polyacrylamide gel electrophoresis in sodium dodecyl sulfate (SDS), and its amino acid composition resembled that reported by others. ApoA-I was iodinated by lactoperoxidase and the resulting 125I-apoA-I was purified by gel filtration. Up to 93% of 125I- apoA-I was precipitable by antibody and greater than 99% of bound 125I- apoA-I was displaced by "cold" apoA-I. Other rat lopoproteins and apolipoproteins did not react in this system. Human plasma were also not reactive, nor were dog, goat, and sheep plasmas.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?