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Journal of Lipid Research, Vol 17, 38-45, Copyright © 1976 by Lipid Research, Inc.

ARTICLES

Measurement of squalene in human tissues and plasma: validation and application

GC Liu, EH Ahrens Jr, PH Schreibman and JR Crouse

A method is described for accurate and reproducible measurement of squalene in plasma, feces, urine, bile, and tissue that depends on isolation by alumina column chromatography after mild saponification and on measurement by gas-liquid chromatography. Recoveries from all tissues exceeded 80% and from plasma 96%; losses were accurately corrected by appropriate additions of squalene as an overall recovery standard.
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