HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Blanchette-Mackie, E. J. Articles by Scow, R. O. Search for Related Content PubMed PubMed Citation Articles by Blanchette-Mackie, E. J. Articles by Scow, R. O. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 17, 57-67, Copyright © 1976 by Lipid Research, Inc.

ARTICLES

Retention of lipolytic products in chylomicrons incubated with lipoprotein lipase: electron microscope study

EJ Blanchette-Mackie and RO Scow

Early effects of lipolysis on the structure of chylomicrons in vitro were studied in rat chylomicrons incubated with purified bovine mild lipoprotein lipase at pH 8.1. The amount of the albumin added to the incubation medium was limited so that free fatty acids (FFA) and partial glycerides formed during lipolysis would accumulate in the chylomicrons. The structures visualized in lipolyzed chylomicrons was found to be affected by pH during preparation of specimens for microscopy, whether fixed with OsO4 and sectioned, or stained with sodium phosphotungstate and examined as whole mounts. Circular aqueous spaces were present in the triglyceride core of lipolyzed chylomicrons processed at pH 8.1 and 7.4. Sometimes the spaces contained aggregates of osmiophilic material and whorls of bilayered lamellae. The spaces were replaced by lamellar structures having a periodicity of 40 A, in chylomicrons processed at pH 5.5, and the spaces and lamellae were both absent at pH 3.0. The findings indicate that these spaces were lined by a lipid monolayer which formed bilayered lamellae under certain conditions. It is concluded that the monolayer lining the aqueous spaces is an inward extension of the chylomicron surface film produced by the accumulation and movement of lipolytic products, FFA and partial glycerides, in the interfacial plane between core triglyceride and water.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Faraj and K. Cianflone Differential regulation of fatty acid trapping in mouse adipose tissue and muscle by ASP Am J Physiol Endocrinol Metab, July 1, 2004; 287(1): E150 - E159. [Abstract] [Full Text] [PDF]

				M. Faraj, A. D. Sniderman, and K. Cianflone ASP enhances in situ lipoprotein lipase activity by increasing fatty acid trapping in adipocytes J. Lipid Res., April 1, 2004; 45(4): 657 - 666. [Abstract] [Full Text] [PDF]

				C. L. Bisgaier, A. D. Essenburg, B. C. Barnett, B. J. Auerbach, S. Haubenwallner, T. Leff, A. D. White, P. Creger, M. E. Pape, T. J. Rea, et al. A novel compound that elevates high density lipoprotein and activates the peroxisome proliferator activated receptor J. Lipid Res., January 1, 1998; 39(1): 17 - 30. [Abstract] [Full Text]