Journal of Lipid Research, Vol 17, 273-277, Copyright © 1976 by Lipid Research, Inc.
Quantitative analysis of individual bile acids by gas-liquid chromatography: an improved method
DC Jones, GW Melchior and MJ Reeves
The quantitative analysis of individual bile acids by gas-liquid
chromatography has been improved by column oven temperature programming and
by a new liquid phase, SP-2401. The method is fast; bile acids are well
resolved; retention times are reproducible; detector responses are linear
and sensitive to 0.1 mug: and there is little adsorption onto the liquid
phase. The method has been successfully used for bile, and it has the
potential for use on serum.