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Journal of Lipid Research, Vol 17, 277-281, Copyright © 1976 by Lipid Research, Inc.
JD McGarry and DW Foster
The radioisotopic assay for carnitine first described by Cederblad and
Lindstedt (Clin. Chim. Acta. 37:235-543, 1972) and modified by Bohmer et al
(Clin. Chim. Acta. 57:55-61, 1974) has been improved and simplified. As a
result, the assay yields a linear response over a wide range of carnitine
concentrations without the need for excessive amounts of labeled
acetyl-CoA. In addition, it will measure very small quantities of carnitine
even in the presence of excess acylcarnitine. The method allows rapid
determination of free and esterified carnitine in small volumes of plasma
(50 mul is sufficient) without the need for prior deproteinization of the
samples.
ARTICLES
An improved and simplified radioisotopic assay for the determination of free and esterified carnitine
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