Journal of Lipid Research, Vol 17, 290-293, Copyright © 1976 by Lipid Research, Inc.
Preparative isolation of cerebrosides (galactosyl and glucosyl ceramide)
NS Radin
An improved method for isolating cerebrosides from natural sources is
described. The method is particularly suited to large scale work and can be
adapted to the isolation of sphingolipids that are less polar than the
gangliosides. It is based on the use of sodium sulfate to absorb the water
from chloroform-methanol tissue extracts, the use of triiodide to cleave
the ether linkage of plasmalogens, and the use of alkaline methanolysis to
cleave the ester linkages of the glycerolipids. The final separation of the
lipids is done with a silica gel column.
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