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Journal of Lipid Research, Vol 17, 294-297, Copyright © 1976 by Lipid Research, Inc.

ARTICLES

The metabolism of 3alpha, 7alpha, 12alpha-trihydroxy-5beta-cholestan-26- oic acid into cholic: an enzyme assay using homogenates of human liver

RF Hanson, HL Sharp and GC Williams

An enzyme assay was developed to measure the conversion of the bile acid precursor, 3alpha, 7alpha, 12alpha-trihydroxy-5beta-cholestan-26- oic acid (THCA), into cholic acid using homogenates of human liver biopsies. The average rate of metabolism of THCA into cholic acid was found to be 3.9 +/- 0.5 (+/- 1 SD) pmoles of cholic acid formed/mg liver/minute in twelve normal liver biopsies. This assay system can be used to determine if the syndrome of neonatal cholestasis associated with a metabolic block in the conversion of THCA into cholic acid is transmitted as a genetic trait.
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