| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Lipid Research, Vol 17, 506-515, Copyright © 1976 by Lipid Research, Inc.
BG Rao and MW Spence
A hitherto undescribed sphingomyelinase (sph'ase 7.4) of human brain has
been studied in crude and partially purified (3- to 4- fold) extracts of
grey matter, and compared to the known sphingomyelinase with an acid pH
optimum (sph'ase 5.0). Its specificity for sphingomyelin as substrate is
similar to that of sph'ase 5.0, but it differs from sph'ase 5.0 in its pH
optimum (7.4 vs 5.0) and in a requirement for Mg2+ for optimal activity.
Other properties of sph'ase 7.4 that distinguish it from sph'ase 5.0
include (a) its lack of appreciable solubilization during dialysis of crude
homogenates (b) a more marked concentrations in grey matter than in white
matter (9- to 13- fold vs 1.5- to 2-fold for sph'ase 5.0); (c) inhibition
by Ca2+ and Cd2+ ions, and by EDTA; (D) stimulation by dithiothreitol, and
inhibition by cysteine, N-ethylmaleimide, and p-hydroxymercuribenzoate; (e)
lack of inhibition by nucleotides (AMP.ADP, and ATP) and by NAD plus NADH;
and (f) relative instability to storage or manipulation between -20degrees
C and 40degrees C. These differences indicate the SPH'ASE 7.4 is a
different enzyme protein from sph'ase 5.0. Unlike sph'ase 5.0, which is
widely distributed in mammalian tissues, sph'ase 7.4 occurs predominantly
in grey matter and little activity was observed is spleen, liver, or
leukocytes. The high levels of this enzyme in brain suggest a role related
to the specific functions of this organ or to the need for a more stringent
control of sphingomyelin catabolism in brain as compared to other organs.
ARTICLES
Sphingomyelinase activity at pH 7.4 in human brain and a comparison to activity at pH 5.0
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Yabu, S. Imamura, M. Yamashita, and T. Okazaki Identification of Mg2+-dependent Neutral Sphingomyelinase 1 as a Mediator of Heat Stress-induced Ceramide Generation and Apoptosis J. Biol. Chem., October 31, 2008; 283(44): 29971 - 29982. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Zumbansen and W. Stoffel Neutral Sphingomyelinase 1 Deficiency in the Mouse Causes No Lipid Storage Disease Mol. Cell. Biol., June 1, 2002; 22(11): 3633 - 3638. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Bernardo, O. Krut, K. Wiegmann, D. Kreder, M. Micheli, R. Schafer, A. Sickman, W. E. Schmidt, J. M. Schroder, H. E. Meyer, et al. Purification and Characterization of a Magnesium-dependent Neutral Sphingomyelinase from Bovine Brain J. Biol. Chem., March 10, 2000; 275(11): 7641 - 7647. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tomiuk, M. Zumbansen, and W. Stoffel Characterization and Subcellular Localization of Murine and Human Magnesium-dependent Neutral Sphingomyelinase J. Biol. Chem., February 25, 2000; 275(8): 5710 - 5717. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Cifone, G. Migliorati, R. Parroni, C. Marchetti, D. Millimaggi, A. Santoni, and C. Riccardi Dexamethasone-Induced Thymocyte Apoptosis: Apoptotic Signal Involves the Sequential Activation of Phosphoinositide-Specific Phospholipase C, Acidic Sphingomyelinase, and Caspases Blood, April 1, 1999; 93(7): 2282 - 2296. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Liu, D. F. Hassler, G. K. Smith, K. Weaver, and Y. A. Hannun Purification and Characterization of a Membrane Bound Neutral pH Optimum Magnesium-dependent and Phosphatidylserine-stimulated Sphingomyelinase from Rat Brain J. Biol. Chem., December 18, 1998; 273(51): 34472 - 34479. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Schissel, G. A. Keesler, E. H. Schuchman, K. J. Williams, and I. Tabas The Cellular Trafficking and Zinc Dependence of Secretory and Lysosomal Sphingomyelinase, Two Products of the Acid Sphingomyelinase Gene J. Biol. Chem., July 17, 1998; 273(29): 18250 - 18259. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Hinkovska-Galcheva, L. Kjeldsen, P. J. Mansfield, L. A. Boxer, J. A. Shayman, and S. J. Suchard Activation of a Plasma Membrane-Associated Neutral Sphingomyelinase and Concomitant Ceramide Accumulation During IgG-Dependent Phagocytosis in Human Polymorphonuclear Leukocytes Blood, June 15, 1998; 91(12): 4761 - 4769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Laethem, Y. A. Hannun, S. Jayadev, C. J. Sexton, J. C. Strum, R. Sundseth, and G. K. Smith Increases in Neutral, Mg2+-Dependent and Acidic, Mg2+-Independent Sphingomyelinase Activities Precede Commitment to Apoptosis and Are Not a Consequence of Caspase 3-Like Activity in Molt-4 Cells in Response to Thymidylate Synthase Inhibition by GW1843 Blood, June 1, 1998; 91(11): 4350 - 4360. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. S. David, P. A. Ortiz, T. R. Smith, and J. Turinsky Sphingomyelinase has an insulin-like effect on glucose transporter translocation in adipocytes Am J Physiol Regulatory Integrative Comp Physiol, May 1, 1998; 274(5): R1446 - R1453. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Suchard, V. Hinkovska-Galcheva, P. J. Mansfield, L. A. Boxer, and J. A. Shayman Ceramide Inhibits IgG-Dependent Phagocytosis in Human Polymorphonuclear Leukocytes Blood, March 15, 1997; 89(6): 2139 - 2147. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Chen, M. Nikolova-Karakashian, A. H. Merrill , Jr., and E. T. Morgan Regulation of Cytochrome P450 2C11 (CYP2C11) Gene Expression by Interleukin-1, Sphingomyelin Hydrolysis, and Ceramides in Rat Hepatocytes J. Biol. Chem., October 20, 1995; 270(42): 25233 - 25238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Coroneos, M. Martinez, S. McKenna, and M. Kester Differential Regulation of Sphingomyelinase and Ceramidase Activities by Growth Factors and Cytokines J. Biol. Chem., October 6, 1995; 270(40): 23305 - 23309. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S Mathias, A Younes, C. Kan, I Orlow, C Joseph, and R. Kolesnick Activation of the sphingomyelin signaling pathway in intact EL4 cells and in a cell-free system by IL-1 beta Science, January 22, 1993; 259(5094): 519 - 522. [Abstract] [PDF]
|
|
|
|
|
|
K. Dressler, S Mathias, and R. Kolesnick Tumor necrosis factor-alpha activates the sphingomyelin signal transduction pathway in a cell-free system Science, March 27, 1992; 255(5052): 1715 - 1718. [Abstract] [PDF]
|
|
|
|
|
|
D. Wenger, M Sattler, T Kudoh, S. Snyder, and R. Kingston Niemann-Pick disease: a genetic model in Siamese cats Science, June 27, 1980; 208(4451): 1471 - 1473. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
