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Journal of Lipid Research, Vol 17, 536-541, Copyright © 1976 by Lipid Research, Inc.
P Nilsson-Ehle and MC Schotz
A method is described for the assay of lipoprotein lipase, using a stable,
radioactive substrate emulsion. Fatty acid-labeled trioleoylglycerol was
emulsified by homogenization in glycerol with lecithin as detergent. This
anhydrous emulsion was stable for at least six weeks. Substrate solutions
for enzyme assay were prepared by diluting the emulsion with buffer
containing serum and albumin. The fatty acid produced on hydrolysis was
isolated in a one-step liquid- liquid partition system. Incubations with
extracts of acetone powders from adipose tissue displayed characteristics
of lipoprotein lipase activity, i.e., serum dependence and inhibition by
NaCl and protamine. The method is rapid (less than 1 hour), sensitive and
reproducible, and suitable for routine use.
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A stable, radioactive substrate emulsion for assay of lipoprotein lipase
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