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Journal of Lipid Research, Vol 17, 545-547, Copyright © 1976 by Lipid Research, Inc.

ARTICLES

Silicic acid thin-layer chromatography of conjugated and free bile acids

MN Chavez and CL Krone

The separation of free bile acids, taurine conjugates, and glycine conjugates from one another was achieved by thin-layer chromatography, using a solvent system of isopropanol glacial acetic acid 93:7 in a solvent system of hexane-methylethylketone-glacial acetic acid 56:36:8 (v/v), it was possible to separate cholic, chenodeoxycholic, deoxycholic, and lithocholic acids. Application to a biological bile sample was demonstrated.
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Copyright © 1976 by the American Society for Biochemistry and Molecular Biology.