Journal of Lipid Research, Vol 17, 588-599, Copyright © 1976 by Lipid Research, Inc.
Insulin binding and insulin response of adipocytes from rats adapted to fat feeding
C Ip, HM Tepperman, P Holohan and J Tepperman
The effect of fat feeding on adipocyte insulin binding was examined to
expand a study of adaptive changes in plasma membrane functions. Cells from
rats fed a high fat (L) diet for five to seven days bound less insulin and
showed a decreased response to insulin (glucose oxidation) compared to
those from rats fed a high glucose (G) diet. Both high and low affinity
sites were influenced; the extent of the binding difference increased as
increasing concentrations of insulin were present in the assay medium. Diet
did not change hormone degradation on the capacity of phospholipase C to
increase binding. Concanavalin A effects on fat cells were also decreased
by L diet both in inhibition of insulin binding and its insulin-like effect
on glucose oxidation. Spermine, which had no effect on insulin binding,
also had a smaller insulin-like effect on glucose oxidation by L cells than
by G cells. Serum insulin was significantly lower (30 +/- 3.7 muU/ml) in L
than in G (43 +/- 3.1 muU/ml) groups. Dietary fat produces alterations in
fat cells that decrease insulin binding as a part of a complex overall
adaptation to the diet.
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