J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
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Journal of Lipid Research, Vol 17, 622-628, Copyright © 1976 by Lipid Research, Inc.

ARTICLES

A biosynthetic method for the preparation of high specific activity 32P- labeled phospholipids

WD Marggraf

32P-Labeled phospholipids with specific activities up to 400 mCi/mmole as well as [32P]CDP-choline were prepared by cultivation of mouse fibroblasts or mouse Ehrlich ascites cells in the presence of [32P]orthophosphate. The method was also used to prepare [methyl- 3H]choline-labeled glycerophospholipids from [3H]choline. The yields and the specific activities of the phospholipids were significantly lower when preparations of ox white blood cells were used.
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Copyright © 1976 by the American Society for Biochemistry and Molecular Biology.