Journal of Lipid Research, Vol 17, 629-636, Copyright © 1976 by Lipid Research, Inc.
Metabolism of glycerol monoethers in cultured liver cells and implications for monoglyceride pathways
CG Mackenzie, OK Reiss, E Moritz and JB Mackenzie
A comparative study has been made of the assimilation and metabolism of
rac-1 and 2-[9, 10(-3)H]-octadec-9-enylglycerol in a clone of
epithelial-like cells isolated from rabbit liver. Based on cell protein
content, the free glycerol ether isomers attained equal cellular
concentrations. As shown by isolation and degradation experiments, however,
the incorporation of radioactive 1-monether was appreciably higher than
that of radioactive 2-monoether in both the triacylglycerol and
phospholipid fractions. The 1-monoether, unlike the 2-monether, was also a
significant source of esterified fatty acids in both lipid fractions. In
addition, the 1-monoether, but not the 2-monoether, was an active precursor
of plasmalogens, particularly ethanolamine plasmalogen. In contrast to the
1-monoether, the 2-monoether was a more active precursor of
triacylglycerols than it was of phospholipids. The results indicate that in
the rabbit liver cells the pathway of complex lipid synthesis from
1-monoether was via 1-alkyl-sn-glycerol-3- phosphoric acid and from
2-monoether via 1-alkyl-2-acyl-sn-glycerol.
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