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Journal of Lipid Research, Vol 17, 637-646, Copyright © 1976 by Lipid Research, Inc.

ARTICLES

Aliphatic medium chain tricarboxylic acids in rat urine

B Eliasson, S Lindstedt and G Steen

Three aliphatic tricarboxylic acids have been found in rat urine. They have been identified as 6-carboxy-5-undecenedioic acid, 6-carboxy-5- dodecenedoic acid, and 6-carboxy-5-tridecenedioic acid. The carbon skeleton structure was determined by mass spectra of the hydrogenated methyl esters. The double bond position was determined after osmium tetroxide oxidation followed by trifluoroacetylation and mass spectrometry and by infrared spectrometry. The compounds were present in the urine when the rats were fed on pellets but disappeared when they received sucrose and water. The acids were not present in the pellets, and a metabolic relation to compounds of longer chain length, possibly mycolic acids, is likely.
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