Journal of Lipid Research, Vol 17, 657-662, Copyright © 1976 by Lipid Research, Inc.
Effects of free fatty acids on the enzymic synthesis of diacyl and ether types of choline and ethanolamine phosphoglycerides
A Radominska-Pyrek, J Strosznajder, Z Dabrowiecki, T Chojnacki and LA Horrocks
Activities of ethanolaminephosphotransferases (EC 2.7.8.1) and choline
phosphotransferases (EC 2.7.8.2) in microsomal fractions from brains and
livers of mature rats are increased several fold by the addition of
1,2-diacyl-sn-glycerols or 1-alkyl-2-acyl-sn-clycerols. Oleic acid added
with diacylglycerols stimulated further the synthesis of lecithins by liver
microsomes, confirming the work of Sribney and Lyman (Can J. Biochem. 51:
1479-1486, 1973). With alkylacylglycerols, oleic and stearic acids were
inhibitory and linoleic acid was even more inhibitory for the synthesis of
both 1-alkyl-1-acyl-sn-glycero-3- phosphorylcholines and the corresponding
ethanolamine compounds with microsomes from both tissues. Free fatty acids
without added diglycerides had mixed effects. These results are best
explained by postulating the presence of two isoenzymes each for
ethanolaminephosphotransferase and cholinephosphotransferase of which only
one is affected by free fatty acids. Regulation of the phosphotransferases
by free fatty acids may determine the proportion of CDP-choline and
CDP-ethanolamine used for synthesis of diacyl and alkylacyl types of these
phosphoglycerides.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
