J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
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Journal of Lipid Research, Vol 17, 674-676, Copyright © 1976 by Lipid Research, Inc.

ARTICLES

Artifacts produced during acid-catalyzed methanolysis of sterol esters

JK Kramer and HW Hulan

Sterol esters were transesterified within 1 hr using either acid or base catalysts. Acid-catalyzed methanolysis of sterol esters with HCl, H2SO4, or BF3 leads to the formation of two artifacts derived from the sterol portion of the molecule; they were identified as dehydrated and methoxylated derivatives of sterols. These two artifacts were not produced using a base-catalyzed methanolysis with NaOCH3.
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Copyright © 1976 by the American Society for Biochemistry and Molecular Biology.