Journal of Lipid Research, Vol 18, 93-98, Copyright © 1977 by Lipid Research, Inc.
Stimulatory action of cycloheximide on glucose metabolism in the rat epididymal fat pad
JA Garcia-Sainz, E Pina and V Chagoya de Sanchez
The action of cycloheximide on some parameters of glucose and lipid
metabolism was studied in vitro in epidiymal fat pads from fasted rats.
Incubation of fat pads with cycloheximide (1 mug/ml) for 2 hours resulted
in a two-fold increase in glucose uptake, glucose oxidation, incorporation
of glucose into lipids, and reesterification of free fatty acid. The
increase in glucose oxidation was evident in experiments in which [U-14C],
[1-14C], or [6-14C]glucose was added to the media, but it was absent when
the media were supplemented with pyruvate. Incorporation of glucose into
glycogen and accumulation of lactate in the medium were not seriously
modified by the presence of cycloheximide. The stimulatory effect of
cycloheximide on incorporation of glucose into lipids was absent when
insulin or cortisol was added to the medium. A cycloheximide-mediated
increase in glucose uptake seems to be responsible for the subsequent
changes in glucose metabolism, and would seem to be independent of an
inhibition in protein synthesis; puromycin and actinomycin D did not mimic
the cycloheximide action on glucose incorporation into lipids.
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