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Journal of Lipid Research, Vol 18, 259-263, Copyright © 1977 by Lipid Research, Inc.

ARTICLES

Microdetermination of cholesterol in serum lipoproteins

CC Heuck, A Nothhelfer, H Raetzer and G Schlierf

A two-step procedure for the microdetermination of cholesterol in serum lipoproteins is compared with cholesterol quantitation after density gradient ultracentrifugation. Serum lipoproteins from 10 mul of serum are separated by electrophoresis on agarose and visualized by precipitation with dextran sulfate--CaCl2. The lipoprotein bands are cut off from the plates, the agarose slices are hydrolyzed by gas- liquid chromatography. The comparison between the two procedures reveals satisfactory correlations for beta-and pre-beta-lipoproteins and total serum. There is excellent recovery of cholesterol in fractionated lipoproteins.
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