Journal of Lipid Research, Vol 18, 517-522, Copyright © 1977 by Lipid Research, Inc.
Glycosphingolipids from cultured astroblasts
J Robert, G Rebel and P Mandel
The glycolipids of two clonal lines astroblasts, NN clone and C6 clone,
were studied. Glucosylceramide and lactosylceramide were present at very
low levels in both clones, but the most common myelin glycolipids
galactosylceramide and sulfatide, were not detected. The ganglioside
pattern of these cells was rather simple, with GM3 as main compoenent,
accompained in one clone by GD3. These results are quite different from
those observed on astroglia-enriched fractions isolated from brain. The
fatty acid composition of these glycolipids was studied. Long chain fatty
acids, up to lignoceric acid, were found in appreciable amounts, even in
gangliosides. It is difficult to conclude if these glycolipid patterns are
due to the properties of normal astroblasts, to transformation, or to a
tumoral character of our cell lines.