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Journal of Lipid Research, Vol 18, 544-547, Copyright © 1977 by Lipid Research, Inc.

ARTICLES

Quantitative determination of hepatic and lipoprotein lipase activities from human postheparin plasma

J Boberg, J Augustin, ML Baginsky, P Tejada and WV Brown

A method was developed to separate and quantitatively determine two different triglyceride lipase activities in human postheparin plasma: hepatic triglyceride lipase (H-TGL) and lipoprotein lipase (LPL). Affinity chromatography on heparin-Sepharose columns was used for the separation. Rechromatography of purified H-TGL on heparin-Sepharose resulted in recoveries of 74 and 97% of these enzyme activities, respectively. The analytical errors for the determinations of the two activities were 11.4 and 9.6%, respectively.
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