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Journal of Lipid Research, Vol 18, 704-709, Copyright © 1977 by Lipid Research, Inc.

ARTICLES

A large-scale purification of phosphatidylethanolamine, lysophosphatidylethanolamine, and phosphatidylethanolamine, and phosphatidylcholine by high performance liquid chromatography: a partial resolution of molecular species

RS Fager, S Shapiro and BJ Litman

Egg yolk phospholipids, on a 10 g scale, were resolved by high- performance liquid chromatography on an 8-m silica column with elution by a stepwise chloroformmethanol gradient into homogenous phosphatidylcholine, phosphatidylinositol, phosphatidylethanolamine, and lysophosphatidylethanolamine fractions. Within these fractions, partial resolution on the basis of fatty acyl side chain composition was achieved.
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