Journal of Lipid Research, Vol 18, 745-752, Copyright © 1977 by Lipid Research, Inc.
Puromycin inhibition of cholesterol absorption in the rat
GV Vahouny, M Ito, EM Blendermann, LL Gallo and CR Treadwell
The effect of puromycin on the intestinal absorption of cholesterol has
been studied in rats with indwelling catheters in the left thoracic
lymphatic duct. Puromycin administration to female rats produced a marked
depression of cholesterol absorption under conditions where the absorption
of simultaneously administered fatty acid was also dramatically inhibited.
The same treatment of male rats also produced a significant depression in
cholesterol absorption, but was without effect on absorption of the fatty
acid. Despite the depressions of lipid absorption in puromycin-treated
animals, there was no accumlation of either cholesterol or fatty acid in
the intestinal mucosa of either sex. Actinomycin D treatment of fasting
male and female rats, receiving constant infusions of saline, had no effect
on the rate of lymph production. This suggest that altered lymph production
was not responsible for the depressed lipid absorption observed in fed
animals treated with protein synthesis inhibitors. The selective inhibition
of cholesterol absorption in male rats also precludes the possibility that
the major effect of the inhibitor is on delayed gastric emptying.
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