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Journal of Lipid Research, Vol 18, 777-780, Copyright © 1977 by Lipid Research, Inc.

ARTICLES

A rapid and sensitive radiochemical assay for phosphatidic acid phosphohydrolase activity

TJ Flynn, DS Deshmukh and RA Pieringer

A technique is described for the radiochemical assay of phosphatidic acid phosphohydrolase activity in rat brain. Radiochemically pure 32P- labeled phosphatidic acid of known specific radioactivity and structure, which was biosynthesized in vitro by the diacylglycerol kinase of E. coli, was used as the substrate. As little as 5 microgram of microsomal or mitochondrial protein can be used for the assay, and product formation in the picomole range can be determined accurately. This procedure should be useful in situations where only limited amounts of tissue are available.
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Copyright © 1977 by the American Society for Biochemistry and Molecular Biology.