Journal of Lipid Research, Vol 19, 12-17, Copyright © 1978 by Lipid Research, Inc.
Rapid determination of the molecular weight distribution of total cellular fatty acids using chemical ionization mass spectrometry
RP Schwarzenbach and NS Fisher
A new method for the qualitative and quantitative determination of the
molecular weight distribution of total cellular fatty acids is described.
The method includes a simple extraction-saponification followed by chemical
ionization-mass spectrometric analysis of the saponifiable matter. This
technique requires small quantities of cell material which, combined with
the rapidity and precision of the analysis, makes it attractive to the
biologist interested in changes in the fatty acid composition of growing
cells. As an example, an application of this method to the fatty acid
determination of marine diatoms at different growth stages is presented.
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