Journal of Lipid Research, Vol 19, 94-102, Copyright © 1978 by Lipid Research, Inc.
Comparison of cholesterol turnover by sterol balance and input-output analysis, and a shortened way to estimate total exchangeable mass of cholesterol by the combination of the two methods
P Samuel, S Lieberman and EH Ahrens Jr
Daily turnover of cholesterol obtained by the balance method was compared
to daily input rates calculated by input-output analysis in 43 experiments.
The mean value of input rates for kinetic data of 10.1- 16.4 weeks'
duration (14 experiments) was 1.05 g/day vs. the chemical turnover of 0.94
g/day (difference 10.9 percent). For decay curves of 4.8-9.9 weeks'
duration (29 experiments) the mean results were 1.67 g/day vs. 1.31 g/day,
respectively (difference 20.1 percent). A combination of the balance method
with input-output analysis is proposed to estimate the size of M (minimum
value of the total exchangeable mass of cholesterol) in short-term
experiments. Using this method, the analysis of curves of 10-12 weeks'
duration showed a mean difference of 7.5 percent with the analysis of
curves of 50-66 weeks' duration in 17 patients. However, because of
considerable variations that can occur in individual cases, it is urged
that a standard correction factor not be used, either in estimating
turnover data or M from 10-12 weeks' kinetic data; rather, the proposed
combined method will alert the investigator to the occurrence of discrepant
results.
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