Journal of Lipid Research, Vol 19, 229-236, Copyright © 1978 by Lipid Research, Inc.
Effect of temperature and plasma on the exchange of apolipoproteins and phospholipids between rat plasma very low and high density lipoproteins
S Eisenberg
The effect of temperature and plasma on the exchange of apoprotein C and
phospholipids between VLDL and HDL was studied using 125I-labeled and
32P-labeled VLDL. Temperature affected the exchange of apoproteins and
phospholipids similarly, and both were enhanced by increasing the
temperature of incubation to 20 degrees C and higher. The exchange of apoC
was almost complete within 5 minutes of incubation and was not influenced
by the addition of plasma to the incubation mixture. The exchange of
phospholipids occurred much more slowly and was enhanced 5- to 10-fold in
the presence of plasma. These results indicate that the exchange of both
phospholipids and apoC is dependent on the temperature of the incubation,
but that they exchange independently, at least in part. It is suggested
that the exchange of apoC occurs through dissociation of the apoprotein
from the lipoprotein surface to the water phase, whereas the exchange of
phospholipids is mediated mainly by a carrier molecule present in plasma
and absent in lipoproteins.
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