Journal of Lipid Research, Vol 19, 244-249, Copyright © 1978 by Lipid Research, Inc.
Ruminal hydrogenation of cholesterol
JR Ashes, SK Gulati, LJ Cook, SC Mills and TW Scott
Cholesterol was hydrogenated by anaerobic incubation with sheep rumen fluid
for periods up to 20 hr. The principal product of cholesterol hydrogenation
was identified as coprostanol. Cholesterol could be protected against in
vitro ruminal hydrogenation by encapsulation in a matrix of
formaldehyde-treated casein. Formaldehyde-treated casein- cholesterol
preparations were also shown to be protected against hydrogenation in vivo
and, when supplements containing 1 g per day of protected or unprotected
cholesterol were fed to sheep over a period of 8-9 weeks, there were marked
differences in the plasma cholesterol response. The plasma cholesterol of
the sheep fed protected cholesterol increased by at least 60%. The plasma
cholesterol of the sheep fed unprotected cholesterol also tended to
increase during the first 5 weeks of supplementation but thereafter
declined to almost control levels at 8 weeks.
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