| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Lipid Research, Vol 19, 269-273, Copyright © 1978 by Lipid Research, Inc.
ARTICLES |
SW Cushman and LB Salans
The osmic acid fixation-Coulter electronic counter method described for determining adipose cell size and number in intact adipose tissue fragments has been modified for use with suspensions of isolated rat and human adipose cells. Mean cell sizes in tissue fragments and isolated cell suspensions prepared from the same tissue are virtually identical in rats of various weights. No statistically significant difference in mean adipose cell size between tissue and isolated cell suspension was observed in human adipose tissue although the variability was much greater than in rat tissue. The distribution of cell sizes among replicate samples is more uniform in the isolated cell preparations, possibly reflecting the considerably larger quantities of tissue used in preparing isolated cells than in determining cell size and number directly from tissue fragments. An example of the utility of the modified method during routine metabolic studies with isolated rat epididymal adipose cells is described; isolated cells of increasing size can be obtained from rats of increasing body weight, or from the separated distal and proximal portions of the fat pads of rats of the same weight.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  N. Inoue, N. Yahagi, T. Yamamoto, M. Ishikawa, K. Watanabe, T. Matsuzaka, Y. Nakagawa, Y. Takeuchi, K. Kobayashi, A. Takahashi, et al. Cyclin-dependent Kinase Inhibitor, p21WAF1/CIP1, Is Involved in Adipocyte Differentiation and Hypertrophy, Linking to Obesity, and Insulin Resistance J. Biol. Chem., July 25, 2008; 283(30): 21220 - 21229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Huang, J. S. Gabrielsen, R. C. Cooksey, B. Luo, L. G. Boros, D. L. Jones, H. A. Jouihan, Y. Soesanto, L. Knecht, M. W. Hazel, et al. Increased Glucose Disposal and AMP-dependent Kinase Signaling in a Mouse Model of Hemochromatosis J. Biol. Chem., December 28, 2007; 282(52): 37501 - 37507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sakai, H. Sakaue, T. Nakamura, M. Okada, Y. Matsuki, E. Watanabe, R. Hiramatsu, K. Nakayama, K. I. Nakayama, and M. Kasuga Skp2 Controls Adipocyte Proliferation during the Development of Obesity J. Biol. Chem., January 19, 2007; 282(3): 2038 - 2046. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. McClain, M. Hazel, G. Parker, and R. C. Cooksey Adipocytes with increased hexosamine flux exhibit insulin resistance, increased glucose uptake, and increased synthesis and storage of lipid Am J Physiol Endocrinol Metab, May 1, 2005; 288(5): E973 - E979. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bluher, M.-E. Patti, S. Gesta, B. B. Kahn, and C. R. Kahn Intrinsic Heterogeneity in Adipose Tissue of Fat-specific Insulin Receptor Knock-out Mice Is Associated with Differences in Patterns of Gene Expression J. Biol. Chem., July 23, 2004; 279(30): 31891 - 31901. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bluher, L. Wilson-Fritch, J. Leszyk, P. G. Laustsen, S. Corvera, and C. R. Kahn Role of Insulin Action and Cell Size on Protein Expression Patterns in Adipocytes J. Biol. Chem., July 23, 2004; 279(30): 31902 - 31909. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Carvalho, S. E. Schellhorn, J. M. Zabolotny, S. Martin, E. Tozzo, O. D. Peroni, K. L. Houseknecht, A. Mundt, D. E. James, and B. B. Kahn GLUT4 Overexpression or Deficiency in Adipocytes of Transgenic Mice Alters the Composition of GLUT4 Vesicles and the Subcellular Localization of GLUT4 and Insulin-responsive Aminopeptidase J. Biol. Chem., May 14, 2004; 279(20): 21598 - 21605. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Chen, F. Mauvais-Jarvis, M. Bluher, S. J. Fisher, A. Jozsi, L. J. Goodyear, K. Ueki, and C. R. Kahn p50{alpha}/p55{alpha} Phosphoinositide 3-Kinase Knockout Mice Exhibit Enhanced Insulin Sensitivity Mol. Cell. Biol., January 1, 2004; 24(1): 320 - 329. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. C. Chen and R. V. Farese Jr. Determination of adipocyte size by computer image analysis J. Lipid Res., June 1, 2002; 43(6): 986 - 989. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. D. Klaman, O. Boss, O. D. Peroni, J. K. Kim, J. L. Martino, J. M. Zabolotny, N. Moghal, M. Lubkin, Y.-B. Kim, A. H. Sharpe, et al. Increased Energy Expenditure, Decreased Adiposity, and Tissue-Specific Insulin Sensitivity in Protein-Tyrosine Phosphatase 1B-Deficient Mice Mol. Cell. Biol., August 1, 2000; 20(15): 5479 - 5489. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. LI, K. L. HOUSEKNECHT, A. E. STENBIT, E. B. KATZ, and M. J. CHARRON Reduced glucose uptake precedes insulin signaling defects in adipocytes from heterozygous GLUT4 knockout mice FASEB J, June 1, 2000; 14(9): 1117 - 1125. [Abstract] [Full Text]
|
|
|
|
|
|
S. C. H. Liu, Q. Wang, G. E. Lienhard, and S. R. Keller Insulin Receptor Substrate 3 Is Not Essential for Growth or Glucose Homeostasis J. Biol. Chem., June 18, 1999; 274(25): 18093 - 18099. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Ferrara, T. H. Reynolds, M. J. Zarnowski, J. T. Brozinick Jr., and S. W. Cushman Short-term exercise enhances insulin-stimulated GLUT-4 translocation and glucose transport in adipose cells J Appl Physiol, December 1, 1998; 85(6): 2106 - 2111. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Chen, P. R. Srinivas, L.-N. Cong, Y. Li, G. Grunberger, and M. J. Quon {alpha}2-Heremans Schmid Glycoprotein Inhibits Insulin-Stimulated Elk-1 Phosphorylation, But Not Glucose Transport, in Rat Adipose Cells Endocrinology, October 1, 1998; 139(10): 4147 - 4154. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L.-N. Cong, H. Chen, Y. Li, L. Zhou, M. A. McGibbon, S. I. Taylor, and M. J. Quon Physiological Role of Akt in Insulin-Stimulated Translocation of GLUT4 in Transfected Rat Adipose Cells Mol. Endocrinol., December 1, 1997; 11(13): 1881 - 1890. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zhou, H. Chen, C. H. Lin, L.-N. Cong, M. A. McGibbon, S. Sciacchitano, M. A. Lesniak, M. J. Quon, and S. I. Taylor Insulin Receptor Substrate-2 (IRS-2) Can Mediate the Action of Insulin to Stimulate Translocation of GLUT4 to the Cell Surface in Rat Adipose Cells J. Biol. Chem., November 21, 1997; 272(47): 29829 - 29833. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Okuno, K. Kajiwara, S. Imai, T. Kobayashi, N. Honma, T. Maki, K. Suruga, T. Goda, S. Takase, Y. Muto, et al. Perilla Oil Prevents the Excessive Growth of Visceral Adipose Tissue in Rats by Down-Regulating Adipocyte Differentiation J. Nutr., September 1, 1997; 127(9): 1752 - 1757. [Abstract] [Full Text]
|
|
|
|
|
|
E. Tozzo, L. Gnudi, and B. B. Kahn Amelioration of Insulin Resistance in Streptozotocin Diabetic Mice by Transgenic Overexpression of GLUT4 Driven by an Adipose-Specific Promoter Endocrinology, April 1, 1997; 138(4): 1604 - 1611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Chen, S. J. Wertheimer, C. H. Lin, S. L. Katz, K. E. Amrein, P. Burn, and M. J. Quon Protein-tyrosine Phosphatases PTP1B and Syp Are Modulators of Insulin-stimulated Translocation of GLUT4 in Transfected Rat Adipose Cells J. Biol. Chem., March 21, 1997; 272(12): 8026 - 8031. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. Houseknecht, A. X. Zhu, L. Gnudi, A. Hamann, J. R. Zierath, E. Tozzo, J. S. Flier, and B. B. Kahn Overexpression of Ha-ras Selectively in Adipose Tissue of Transgenic Mice J. Biol. Chem., May 10, 1996; 271(19): 11347 - 11355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. S. Susulic, R. C. Frederich, J. Lawitts, E. Tozzo, B. B. Kahn, M.-E. Harper, J. Himms-Hagen, J. S. Flier, and B. B. Lowell Targeted Disruption of the beta(3)-Adrenergic Receptor Gene J. Biol. Chem., December 8, 1995; 270(49): 29483 - 29492. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
