Journal of Lipid Research, Vol 19, 325-331, Copyright © 1978 by Lipid Research, Inc.
Properties of cholesteryl oleate and triolein in mixed monolayers at the air-water interface
JM Smaby and HL Brockman
The properties of cholesteryl oleate and triolein in mixed monolayers at
the air-water interface have been measured between 24 and 37 degrees C.
Analysis of force-area curves obtained as a function of the mol fraction of
cholesteryl oleate indicates that at relatively low surface pressures these
compounds are miscible in two dimensions up to a limit of about 0.5 mol
fraction. At higher pressures either cholesteryl oleate or both lipids are
expelled from the monolayer to form a bulk phase which is in rapid
equilibrium with the surface phase. In the monolayer phase, orientation of
the ester function of cholesteryl oleate is toward the aqueous phase,
interaction with triolein is minimal, and packing is uniform over the
solubility range. This together with the susceptibility of the cholesteryl
oleate to enzymatic hydrolysis, suggests the applicability of monolayer
systems to the study of cholesterol esterase activity. Comparison of our
results with the bulk properties of these lipids suggests that the expelled
cholesteryl oleate exists as a smectic mesophase and thus the system may
provide a model for studying the transfer of molecules between the interior
and surface of lipid deposits of the type found in atherosclerotic lesions.
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