Journal of Lipid Research, Vol 19, 370-374, Copyright © 1978 by Lipid Research, Inc.
Characterization of trimethylsilyl derivatives of cerebrosides by direct inlet-chemical ionization mass spectrometry
T Murata, T Ariga, M Oshima and T Miyatake
Submicrogram quantities of trimethylsilyl derivatives of cerebrosides
obtained from the spleen of a patient with Gaucher's disease and from
bovine brain were analyzed by direct probe inlet-chemical ionization mass
spectrometry, using isobutane as the reagent gas. Quasimolecular ions (QM+,
M + 73) and other recognizable fragment ions produced by the successive
elimination of trimethylsilanol and sugar residue gave useful information
about fatty acid compositions. These ions could also be utilized for
qualitative analyses of the molecular species of cerebrosides. Cerebrosides
with non-hydroxy and hydroxy fatty acids could be discriminated from each
other by comparing the intensities of their quasimolecular ions.
Cerebrosides with saturated and monounsaturated fatty acids could also be
discrimnated from each other, because the mass number decreased by two mass
units in cerebrosides with monounsaturated fatty acids. It was concluded
that structural information and molecular species determination could be
obtained from small amounts of purified cerebrosides.
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