Journal of Lipid Research, Vol 19, 390-395, Copyright © 1978 by Lipid Research, Inc.
A rapid and quantitative method for the isolation of gangliosides and neutral glycosphingolipids by DEAE-silica gel chromatography
SK Kundu and SK Roy
DEAE-silica gel has been shown to be an improvement over DEAE-Sephadex for
the quantitative isolation of gangliosides and neutral glycosphingolipids
from animal tissues or cells. Preliminary results indicated that it can
also be used for protein separation. Direct comparative studies of
DEAE-silica gel with DEAE-Sephadex showed preferences for the former for
the following reasons: i) faster flow rate; ii) more rapid equilibration
with the starting buffer; iii) easier regeneration; iv) more economical;
and v) a lesser susceptibility to microbial attack.
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