Journal of Lipid Research, Vol 19, 443-447, Copyright © 1978 by Lipid Research, Inc.
Radioimmunoassay of ursodeoxycholic acid in serum
I Makino, A Tashiro, H Hashimoto, S Nakagawa and I Yoshizawa
A sensitivie and specific radioimmunoassay for the measurement of serum
ursodeoxycholic acid has been developed. Ursodeoxycholic acid bound to
bovine serum albumin was used as an antigen, and antiserum to this antigen
was raised in the rabbit. [11, 12-3h2]Ursodeoxycholic acid was used as the
radioactive tracer, and the radioimmunoassay was carried out by the method
of Simmonds et al. (1973. Gastroenterology. 65: 705- 711). The percentage
of bound radioactivity decreased linearly with a logarithmic increase in
unlabeled ursodeoxycholic acid from 10 to 200 pmol. The antiserum showed
extremely high specificity for ursodeoxycholic acid (free and conjugated),
and the values determined by radioimmunoassay indicated a close correlation
with those found by gas-liquid chromatography. In normal Japanese subjects,
a small amount of ursodeoxycholic acid in serum was detected, and the level
was detected, and the level was 0.15 +/- 0.11 nmol/ml. This convenient
radioimmunoassay will provide useful information about the metabolism of
ursodeoxycholic acid in man.
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