Journal of Lipid Research, Vol 19, 505-509, Copyright © 1978 by Lipid Research, Inc.
Vulnerability of keto bile acids to alkaline hydrolysis
G Lepage, A Fontaine and CC Roy
Rigorous alkaline hydrolysis of the two primary (cholic and
chenodeoxycholic) and of the two preponderant secondary (deoxycholic and
lithocholic) bile acids found in bile led to excellent recoveries. Such was
not the case with 11 different keto bile acid standards. Recoveries for a
number of standards were unacceptably low and a variety of artefactural
products were tentatively identified by gas- liquid chromatography. Keto
bile acids bearing a keto gropu on C-3 were particularly vulnerable. In
view of thee findings, quantitative and qualitative data reported on
biological specimens submitted to saponification in ethanol, methanol, or
even in water are of questionable significance.
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