Journal of Lipid Research, Vol 19, 570-577, Copyright © 1978 by Lipid Research, Inc.
ACTH-induced hydrolysis of cholesteryl esters in rat adrenal cells
GV Vahouny, R Chanderbhan, R Hinds, VA Hodges and CR Treadwell
Rat adrenal cortical cells have been prepared by collagenase dissociation
of trypsin-treated adrenal tissue. The content and compositions of
cholesteryl ester, phospholipid, and triglyceride fatty acids compare
favorably with those of undissociated rat adrenal tissue. During 2-hour
control incubations of adrenal cortical cells, steroidogenesis was not
detected, and the levels of sterol ester, phospholipid, and triglyceride
fatty acids were not significantly altered. Incubations with
adrenocorticotropic hormone (ACTH) resulted in coricosterone production and
significant depletions of sterol ester and triglyceride fatty acids, but
not of phospholipid fatty acids. Although all fatty acid esters of
cholesterol were hydrolyzed under these conditions, the greatest
contributions to the net decrease in sterol esters were by oleate,
arachidonate, and adrenate. Incubations with dibutyryl cyclic adenosine
monophosphate (0.5 mM) resulted in significantly greater levels of
corticosterone production than did ACTH (250 muunits), but the effects on
cellular lipids were comparable to those seen with the tropic hormone. This
study represents the first demonstration of hormone-induced hydrolysis of
sterol esters in an in vitro cell suspension system. The results are
discussed with respect to hormone-sensitive sterol ester hydrolase of
adrenal cortex, and to the role of endogenous cholesteryl esters in the
steroidogenic pathway.
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